UV-Visible spectroscopy is a very important techniques to analyse many parameters in biological experimentation like DNA purity, oxidized-reduced state of molecules, presence of a particular molecule etc. As always I have focused more on experimental examples then instrumentation and by watching this video you will learn following concepts –

1. Basic concepts of UV-Visible spectroscopy

2. Importance of double bonds and conjugated system in getting UV-Visible spectrum

3. UV-Visible spectrum of ATP,DNA

4. Analysis of NAD+ to NADH conversion or vice versa by UV-Visible spectrum

5. UV-Visible spectrum of Cytochrome c

6. UV-Visible spectrum of proteins

7. Analysis of DNA melting point by UV-Visible spectroscopy

8. Analysis or protein or DNA purity by UV-Visible spectroscopy

Circular Dichroism (CD) spectroscopy

1. Basic concept

2. How to measure CD and Cd spectroscopy

3. Examples of CD spectroscopy