Experimental Methods in Systems Biology
Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging.A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content.
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Syllabus
Syllabus - What you will learn from this course
Week 1
Introduction
Week 2
Deep mRNA Sequencing
Week 3
Mass Spectrometry-Based Proteomics
Week 4
Midterm Exam
Week 5
Flow and Mass Cytometry for Single Cell Protein Levels and Cell Fate
Week 6
Live-cell Imaging for Single Cell Protein Dynamics
Week 7
Integrating and Interpreting Datasets with Network Models and Dynamical Models
Week 8
Final Exam
FAQ
When will I have access to the lectures and assignments?
Access to lectures and assignments depends on your type of enrollment. If you take a course in audit mode, you will be able to see most course materials for free. To access graded assignments and to earn a Certificate, you will need to purchase the Certificate experience, during or after your audit. If you don't see the audit option:
The course may not offer an audit option. You can try a Free Trial instead, or apply for Financial Aid.
The course may offer 'Full Course, No Certificate' instead. This option lets you see all course materials, submit required assessments, and get a final grade. This also means that you will not be able to purchase a Certificate experience.
What will I get if I subscribe to this Specialization?
When you enroll in the course, you get access to all of the courses in the Specialization, and you earn a certificate when you complete the work. Your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.
Is financial aid available?
Yes. In select learning programs, you can apply for financial aid or a scholarship if you can’t afford the enrollment fee. If fin aid or scholarship is available for your learning program selection, you’ll find a link to apply on the description page.
Reviews
Un excelente curso que da un panorama muy útil de las herramientas comúnmente empleadas para experimentos enfocados a la biología de sistemas
Amazing course, only the final exam was rather an excercise in finding out out where the answers differ than trying to really understand what should be the ringht answer. I learnt a lot, thank you!
This course is definitely not for beginners and students must have previous lab experience (in my opinion). It is a great course to get your feet wet in new techniques that you have not performed yet.
excellent overview of experimental methods in systems biology
